Functional Reconstitution of the Phagocyte NADPH Oxidase by Transfection of Its Multiple Components in a Heterologous System
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چکیده
The phagocyte NADPH oxidase system, as previously defined by cell-free reconstitution, is comprised of five essential components, three of which are produced during late phagocytic differentiation-namely, two cytosolic proteins, p47and p67-phox-and the large subunit of cytochrome bSs8, gp91-phox. To confirm that these are the only phagocyte-specific components necessary for oxidase activity in whole cells, the recombinant NADPH oxidase was reconstituted in aheterologous cell ine. An undifferentiated multipotent leukemic cell line, K562, which expresses endogenous Rac and the small subunit of the flavocytochrome bW (p22-phoxl, was cotransfected with episomal expression vectors containing cDNAs for the three other oxidase components. After 4 days of selection, the complete oxidase
منابع مشابه
Functional reconstitution of the phagocyte NADPH oxidase by transfection of its multiple components in a heterologous system.
The phagocyte NADPH oxidase system, as previously defined by cell-free reconstitution, is comprised of five essential components, three of which are produced during late phagocytic differentiation--namely, two cytosolic proteins, p47- and p67-phox--and the large subunit of cytochrome b558, gp91-phox. To confirm that these are the only phagocyte-specific components necessary for oxidase activity...
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